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Chapters
- 00:00Title
- 01:46Introduction
- 03:04Calibrating the Spectrally Resolved Two-photon Microscope
- 08:58Collecting Data on Biological Samples of Interest
- 12:36Protein Interactions Determined by Spectrally Resolved Two-photon Microscopy
- 14:03Conclusion
Empregando um espectralmente resolvido dois fótons sistema de imagens de microscopia, pixel-nível mapas de Förster Resonance Energy Transfer (FRET) eficiências são obtidas para células que expressam receptores de membrana para formar a hipótese de homo-oligoméricos complexos. Da FRET mapas eficiência, somos capazes de estimar informações sobre o complexo estequiométrico oligômero em estudo.
Tags
In Vivo QuantificationG Protein Coupled Receptor InteractionsSpectrally Resolved Two-photon MicroscopyProtein InteractionsFRETFluorescent ProbesLiving CellsIndustrial ApplicationsBasic Biological KnowledgeDonor MoleculeAcceptor MoleculeLaser LightFluorescence EmissionSpectral PropertiesProtein Interactions DegreeCell ScanningMolecular CompositionAcquisition ProcessSpatial DeterminationQuantitative ValuesSpectrally Resolved Two-photon Microscope
