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Chapters
- 00:05Title
- 02:23Performing the Capture Assay and Controls
- 08:26Tryptic Digest of Captured Proteins and Preparation of Peptides for LC-MS/MS
- 09:50Peptide and Protein Identification via Automated Sequence Database Search
- 12:33Representative Results of CCMS Experiments
- 16:00Conclusion
Composti cattura sono trifunzionale piccole molecole per ridurre la complessità del proteoma funzionale reversibile da piccola molecola-proteina seguiti da foto-reticolazione e purificazione. Qui si usa un composto di cattura con<em> S</em>-Adenosil-<sub> L</sub>-Omocisteina vincolante in quanto la funzione di isolare la selettività metiltransferasi da un<em> Escherichia coli</emCellulari> intero lisato e identificarli da MS.
Tags
MethyltransferasesS-adenosyl-L-homocysteine-binding ProteinsCapture Compound Mass SpectrometryAffinity ChromatographyActivity-based Protein ProfilingTrifunctional Capture CompoundsPhoto-crosslinkingSmall Molecule-protein InteractionsSelectivity FunctionSAHSorting FunctionBiotinSolid PhaseStreptavidin Magnetic BeadsOff-bead ConfigurationOn-bead ConfigurationSmall Molecule Of InterestSAMSAM-dependent MethyltransferasesDNA Methylation
