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使用胚体冰冻切片分析的多能干细胞
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Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
Please note that all translations are automatically generated.
Click here for the English version.
使用胚体冰冻切片分析的多能干细胞
DOI:
10.3791/2344-v
•
06:54 min
•
December 08, 2010
•
Ismael C. Gomes*
1
,
Mariana Acquarone*
1
,
Renata de Moraes Maciel*
1
,
Rafael Bierig Erlich
,
Stevens K. Rehen
1
Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas
,
Universidade Federal do Rio de Janeiro (UFRJ), Brazil
Chapters
00:02
Title
00:07
Introduction
00:37
Materials and Equipment
01:09
Preparing Embryoid Body Cryo-Blocks
05:14
Cutting
06:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
多能干细胞悬浮液中生长分化成胚状体(EBS)。在这里,我们展示了如何获得高品质的EB冰冻切片,用于研究胚胎发育的细胞和分子方面,同时保留其作为集合体的组织。
Tags
Pluripotent Stem Cells
Cryosections
Embryoid Bodies
ES Cells
Differentiation
Aggregates
Ectoderm
Mesoderm
Endoderm
Immunofluorescence
In Situ Hybridization
Target Proteins
MRNA
Cryosection Technique
Analytical Procedures
Histology Staining Analysis
Hematoxilin And Eosin
DAPI
Oct4
Nestin
Embryogenesis
Tri-dimensional Spherical Structure
Article
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