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使用胚体冰冻切片分析的多能干细胞
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
DOI:

06:54 min

December 08, 2010

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Chapters

  • 00:02Title
  • 00:07Introduction
  • 00:37Materials and Equipment
  • 01:09Preparing Embryoid Body Cryo-Blocks
  • 05:14Cutting
  • 06:37Conclusion

Summary

Automatic Translation

多能干细胞悬浮液中生长分化成胚状体(EBS)。在这里,我们展示了如何获得高品质的EB冰冻切片,用于研究胚胎发育的细胞和分子方面,同时保留其作为集合体的组织。

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