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Percoll 그라디언트를 사용하여 두뇌와 척수 Mononuclear 세포의 분리
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
DOI:

09:58 min

February 02, 2011

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Chapters

  • 00:05Title
  • 01:27Preparation of Reagents
  • 02:09Tissue Collection and Homogenization
  • 02:56Gradient Set Up
  • 05:56Antibody Staining
  • 08:08Representative Analysis by Flow Cytometry
  • 09:29Conclusion

Summary

Automatic Translation

현재 문서는 효율적으로 효과적으로 흐름 cytometric 분석을 위해 활용할 수 뇌 및 척수 조직에서 mononuclear 세포를 분리하기 위해 빠른 프로토콜을 설명합니다.

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