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大脑和脊髓的单核细胞的分离,使用Percoll梯度
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
DOI:

09:58 min

February 02, 2011

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Chapters

  • 00:05Title
  • 01:27Preparation of Reagents
  • 02:09Tissue Collection and Homogenization
  • 02:56Gradient Set Up
  • 05:56Antibody Staining
  • 08:08Representative Analysis by Flow Cytometry
  • 09:29Conclusion

Summary

Automatic Translation

本文介绍一个快速的协议,有效地从大脑和脊髓组织,可以有效地利用流式细胞仪分析中分离出的单个核细胞。

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