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Immunology and Infection
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监测后腹腔感染的免疫细胞贩运荧光朊病毒棒时间
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
Please note that all translations are automatically generated.
Click here for the English version.
监测后腹腔感染的免疫细胞贩运荧光朊病毒棒时间
DOI:
10.3791/2349-v
•
09:22 min
•
November 19, 2010
•
Theodore E. Johnson*
1
,
Brady A. Michel*
1
,
Crystal Meyerett
,
Angela Duffy
,
Anne Avery
,
Steven Dow
,
Mark D. Zabel
1
Department of Microbiology, Immunology and Pathology
,
Colorado State University
Chapters
00:00
Title
01:12
Purifying and Labeling Prion Rods
03:37
Prion Inoculation and Cell Recovery
06:51
Cell Staining and Flow Cytometry
08:09
Cellular Trafficking Response to Purified Prion Rods
08:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种新的分析监测朊蛋白的吸收和免疫细胞的活动,通过净化后立即腹腔注射和荧光标记从被感染的大脑,然后监测其吸收和运动从注射部位和调解这些事件的细胞特征的材料汇总朊病毒棒。
Tags
Immune Cells
Trafficking
Fluorescent Prion Rods
Intraperitoneal Infection
Abnormal Form
Host-encoded Prion Protein (PrPC)
Protease Resistant
Pathologic
Infectious
Prion Diseases
Chronic Wasting Disease (CWD)
Scrapie
Prion Hypothesis
Immune System
Peripheral Prion Pathogenesis
Complement System
Dendritic Cells
Macrophages
Prion Uptake
Trafficking
Inoculation Site
Article
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