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January 26, 2011
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Summary
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クラスリン依存性エンドサイトーシスは、貨物の選択とクラスリン被覆のアセンブリを調整するアダプタータンパク質に依存します。ここではモデルとして酵母エンドサイトーシスのアダプタータンパク質のSla1pを使用してアダプタクラスリンの物理的相互作用および生細胞イメージングのアプローチを研究するための手順を説明します。
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Cite this Article
Feliciano, D., Bultema, J. J., Ambrosio, A. L., Di Pietro, S. M. In vivo and in vitro Studies of Adaptor-clathrin Interaction. J. Vis. Exp. (47), e2352, doi:10.3791/2352 (2011).
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