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Journal
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Neuroscience
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生成高度丰富的神经文化皮层组织块冷冻保存
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal
Neuroscience
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
Please note that all translations are automatically generated.
Click here for the English version.
生成高度丰富的神经文化皮层组织块冷冻保存
DOI:
10.3791/2384-v
•
09:13 min
•
November 11, 2010
•
Ardeshir S. Rahman
,
Shaudee Parvinjah
,
Michael A. Hanna
,
Pablo R. Helguera
,
Jorge Busciglio
1
Department of Neurobiology and Behavior
,
University of California, Irvine
Chapters
00:00
Title
01:15
Material Preparation
02:41
Tissue Preparation
04:20
Cryopreservation
06:05
Thaw and Culture
08:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一个高效的冷冻保存的方法和解冻皮质的脑组织块生成神经元文化的高度浓缩。这个简单的协议提供了神经元,星形胶质细胞和神经元前体细胞培养后一代的灵活性。
Tags
Cryopreservation
Neuronal Cultures
Freezing Medium
Dimethyl Sulfoxide (DMSO)
Hank’s Buffered Salt Solution (HBSS)
Cryovials
Rate-controlled Freezing Container
Post-thaw Processing
Dissociation
Neuronal-enriched Cultures
Neuritic Growth
Immunocytochemical Staining
Astrocytic Marker
Glial Fibrillary Acidic Protein (GFAP)
Neuronal Marker
Beta-tubulin Class III
Neural Precursor Cell Cultures
Neurospheres
Differentiating Conditions
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