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一个Biomining从宏基因组库的纤维素酶的活性高通量筛选
JoVE Journal
Biology
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JoVE Journal
Biology
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Please note that all translations are automatically generated.
Click here for the English version.
一个Biomining从宏基因组库的纤维素酶的活性高通量筛选
DOI:
10.3791/2461-v
•
10:21 min
•
February 01, 2011
•
Keith Mewis
,
Marcus Taupp
,
Steven J. Hallam
1
Microbiology and Immunology
,
University of British Columbia – UBC
Chapters
00:05
Title
01:05
Replication of the Metagenomic Library Plates
03:39
Measuring the Growth of E. coli Clones.
06:35
Addition of the Assay Mix to Each Plate
07:47
Reading Absorbance of Assayed Clones
09:17
Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
09:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种在大肠杆菌中表达了宏基因组文库的纤维素活动的高通量筛选。屏幕解决方案的基础和高度自动化,并采用一锅煮化学与最后一个测吸光度读数在384孔板。
Tags
High Throughput Screen
Biomining Cellulase Activity
Metagenomic Libraries
Cellulose
Industrial Applications
Biofuel Production
Chemical Methods
Enzymatic Methods
Cellulases
Bacterial Isolates
Fungal Isolates
Laboratory Cultivation
Environmental Genomic Screening
Metagenomic Screening Approaches
Soils
Buffalo Rumen
Termite Hind-gut
Carboxymethylcellulose (CMC) Agar Plates
Congo Red Dye
Method Of Teather And Wood
Signal To Noise Ratio
Chromogenic Dinitrophenol (DNP)-cellobioside Substrate
PCC1 C
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