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一个Biomining从宏基因组库的纤维素酶的活性高通量筛选
JoVE Journal
Biology
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JoVE Journal Biology
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
DOI:

10:21 min

February 01, 2011

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Chapters

  • 00:05Title
  • 01:05Replication of the Metagenomic Library Plates
  • 03:39Measuring the Growth of E. coli Clones.
  • 06:35Addition of the Assay Mix to Each Plate
  • 07:47Reading Absorbance of Assayed Clones
  • 09:17Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
  • 09:56Conclusion

Summary

Automatic Translation

该协议描述了一种在大肠杆菌中表达了宏基因组文库的纤维素活动的高通量筛选。屏幕解决方案的基础和高度自动化,并采用一锅煮化学与最后一个测吸光度读数在384孔板。

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