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Chapters
- 00:05Title
- 01:05Replication of the Metagenomic Library Plates
- 03:39Measuring the Growth of E. coli Clones.
- 06:35Addition of the Assay Mix to Each Plate
- 07:47Reading Absorbance of Assayed Clones
- 09:17Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
- 09:56Conclusion
Este protocolo descreve um ecrã de alta taxa de transferência para a atividade celulolítica de uma biblioteca metagenomic expressa em Escherichia coli. A tela é solução baseada e altamente automatizado, e usa um pot-química em 384 microplacas bem com a leitura final como uma medida de absorbância.
Tags
High Throughput ScreenBiomining Cellulase ActivityMetagenomic LibrariesCelluloseIndustrial ApplicationsBiofuel ProductionChemical MethodsEnzymatic MethodsCellulasesBacterial IsolatesFungal IsolatesLaboratory CultivationEnvironmental Genomic ScreeningMetagenomic Screening ApproachesSoilsBuffalo RumenTermite Hind-gutCarboxymethylcellulose (CMC) Agar PlatesCongo Red DyeMethod Of Teather And WoodSignal To Noise RatioChromogenic Dinitrophenol (DNP)-cellobioside SubstratePCC1 C
