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Chapters
- 00:05Title
- 01:05Replication of the Metagenomic Library Plates
- 03:39Measuring the Growth of E. coli Clones.
- 06:35Addition of the Assay Mix to Each Plate
- 07:47Reading Absorbance of Assayed Clones
- 09:17Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
- 09:56Conclusion
Detta protokoll beskriver en hög genomströmning skärm för cellulolytic aktivitet från en metagenomic bibliotek uttryckt i Escherichia coli. Skärmen är baserad och högt automatiserad, och använder ett-gryta kemi i 384 väl mikroplattor med den slutliga avläsningen som en absorbans mätning.
Tags
High Throughput ScreenBiomining Cellulase ActivityMetagenomic LibrariesCelluloseIndustrial ApplicationsBiofuel ProductionChemical MethodsEnzymatic MethodsCellulasesBacterial IsolatesFungal IsolatesLaboratory CultivationEnvironmental Genomic ScreeningMetagenomic Screening ApproachesSoilsBuffalo RumenTermite Hind-gutCarboxymethylcellulose (CMC) Agar PlatesCongo Red DyeMethod Of Teather And WoodSignal To Noise RatioChromogenic Dinitrophenol (DNP)-cellobioside SubstratePCC1 C
