JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
使用内源性野生型BRCA1基因的细胞表达,确定同源重组的BRCA1基因突变的影响
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
Please note that all translations are automatically generated.
Click here for the English version.
使用内源性野生型BRCA1基因的细胞表达,确定同源重组的BRCA1基因突变的影响
DOI:
10.3791/2468-v
•
08:53 min
•
February 17, 2011
•
Jeffrey Parvin
,
Natsuko Chiba
,
Derek Ransburgh
1
Department of Biomedical Informatics
,
The Ohio State University
,
2
Departments of Molecular Immunology and Clinical Oncology
,
Tohoku University
Chapters
00:05
Title
02:01
Preparation of Cell Line with an Integrated Recombination Substrate
03:04
Transfection to Deplete Endogenous BRCA1 and add back Mutant BRCA1
05:19
Analysis of Transfectants
06:07
Analysis of the Effects of BRCA1 Mutations on Homologous Recombination via GFP-Expression
07:54
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们提供了一个用于测试在一个组织文化同源重组修复DNA损伤的检测消耗使用RNA干扰细胞内源性BRCA1蛋白和替换它与BRCA1基因点突变,其中包含一个编码变化的BRCA1基因变体的方法。
Tags
BRCA1 Mutations
Homologous Recombination
Endogenous BRCA1
Missense Mutations
Functional Analysis
Cell Lines
RNAi Targeting
BRCA1 MRNA
BRCA1 Variant
Acute Silencing
Secondary Mutations
Compensation For Loss Of Function
HeLa-derived Cell Line
Homologous Recombination Assay
Recombination Substrate
I-SceI Restriction Enzyme Site
GFP Allele
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用丙烯酰胺水凝胶的细胞功能的研究矩阵刚度的影响
为极化上皮细胞注射质粒的小GTP酶的功能分析
一个基于PCR的基因分型方法来区分野生型和观赏品种
白茅</em
重组工程同源重组构建<em>果蝇</em
测量细菌的影响<em> C。线虫</em>行为使用一个鸡蛋保留含量
基因组DNA富集转座为基础的技术的整个序列的农工商分析<em> BRCA1基因</em><em> BRCA2</em>和9个基因参与DNA损伤修复
鉴定蛋白质相互作用的网站使用多肽阵列
测定野生型肝脏和牛奶样品中的唾液酸<em> CMAH</em>敲出小鼠
评估细菌素对小鼠肠道微生物的影响的方法
用活细胞显微镜研究低压等离子体灭菌对
枯草芽孢杆菌
孢子存活的不利影响
Read Article