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Chapters
- 00:05Title
- 01:08Introduction
- 02:00The Creation of Virtual Slices of Immunohistochemically Stained Images
- 04:15Computer-Assisted Two-Dimensional Analysis
- 05:42Three-Dimensional Reconstruction of Two Dimensional Immunohistochemically Stained Virtual Slice Images
- 07:25Islet Mapping: Collecting Image Stacks
- 10:21Islet Mapping: Mapping Image Stacks
- 12:36Representative Results for the Large-Scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
- 15:47Conclusion
Méthodes assistées par ordinateur du roman achats à grande échelle et d'analyse des échantillons pancréatiques immunohistochimiquement colorées sont décrites: (1) la capture Slice virtuelle de toute la section, l'analyse de masse (2) de données à grande échelle; (3) la reconstruction de la 2D tranches virtuelles (4); cartographie des îlots 3D, et (5) L'analyse mathématique.
Tags
Computer-assistedLarge-scale VisualizationQuantificationPancreatic IsletIslet MassSize DistributionArchitectureEndocrine CellsBeta-cellsAlpha-cellsDelta-cellsPancreas WeightTotal Beta-cell MassIslet Size DistributionFunctional LimitLarge Animal PancreataIslet PlasticityCellular CompositionArchitectureSpecies Variation
