JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Biology
/
收集可变浓度等温滴定量热数据集,以确定约束机制
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms
Please note that all translations are automatically generated.
Click here for the English version.
收集可变浓度等温滴定量热数据集,以确定约束机制
DOI:
10.3791/2529-v
•
10:04 min
•
April 07, 2011
•
Lee A. Freiburger
,
Anthony K. Mittermaier
,
Karine Auclair
1
Department of Chemistry
,
McGill University
Chapters
00:05
Title
01:10
Introduction
01:28
Preparation of Stock Solutions
03:29
Prepare the Isothermal Titration Calorimetry Samples
04:48
Syringe Set-up
05:33
Load the Sample Cell
06:54
Load the Injection Syringe and Initiate the Run
08:57
Variable Concentration Isotherms and Binding Modeling
09:46
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ITC是一个功能强大的工具,为研究其主机的一个配体的结合。然而,在复杂的系统的几种模式可能适合同样的数据。这里介绍的方法,提供了一种手段来澄清合适的绑定模型和复杂的系统,提取相应的热力学参数。
Tags
Isothermal Titration Calorimetry
ITC
Thermodynamic Parameters
Ligand
Host Macromolecule
Binding Enthalpy
Association Constant
C-value
Mechanistic Model
Protein Concentration
Ligand Binding Sites
Multiple Binding Sites
Experimental Data
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
人类冠状病毒的滴定使用免疫过氧化物酶含量
在手术伤口的痛觉及炎症介质的收集和测量
蛋白酶荧光检测试剂盒,用它来确定蛋白酶活性
PAR剪辑 - 一个方法来确定全转录组学,RNA结合蛋白的结合位点
用于测量高分子配体亲和等温滴定量热
原代培养大鼠内髓集合管细胞
糖原生化滴定<em>在体外</em
相关的mRNA分离与酵母线粒体研究本地化翻译的机制
测量外产酸和分析,以确定糖酵解率
肠上皮细胞通透性
的体外
及
体内
测定方法
Read Article