Journal
/
Neuroscience
/
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

DOI:
10.3791/2564-v

•

11:57 min

•

May 17, 2011

•

, , ,
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Clayton Foundation Laboratories for Peptide Biology,The Salk Institute for Biological Studies

Chapters

  • 00:05Title
  • 02:09Preparation of Organotypic Cerebellar Cultures
  • 05:53Fas Treatment and Staining of Cerebellar Slices
  • 09:49Examples of Cerebellar Slice Cultures
  • 10:49Conclusion

Summary

Automatic Translation

Automatic Translation

This method describes the generation of organotypic cerebellar cultures and the effect of certain apoptotic stimuli on the viability of different cerebellar cell types.

Tags

Organotypic Cerebellar CulturesApoptotic ChallengesDetectionNeuroscienceOrganotypic CulturesPreparationSlicesStaining ProcedureBrain PartsHippocampusCerebellumCellular OrganizationNeuronal NetworksPurkinje CellsDissociated Primary CultureDevelopmental FeaturesIn Vitro StudiesElectrophysiological ExperimentsPharmacological ExperimentsApoptotic StimuliFas LigandTUNEL StainingCell Death Measurement

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2564?article_type=v

Organotypic Hippocampal Slice Cultures
check_url/2564?article_type=v

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
check_url/2564?article_type=v

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
check_url/2564?article_type=v

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
check_url/2564?article_type=v

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
check_url/2564?article_type=v

The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
check_url/2564?article_type=v

Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester
check_url/2564?article_type=v

Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays
check_url/2564?article_type=v

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
check_url/2564?article_type=v

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Read Article