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单粒子电子显微镜外切体综合重建,使用随机的锥形倾斜方法
JoVE Journal
Biology
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JoVE Journal Biology
Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
DOI:

12:10 min

March 28, 2011

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Chapters

  • 00:05Title
  • 01:11Principle of the Random Conical Tilt Method
  • 02:10Preparation of Holey Carbon Grids Covered with Thin Carbon
  • 05:08Negative Staining of the Exosome Complex
  • 06:23Electron Microscopy of the Exosome Complex
  • 08:10Image Processing of the Data
  • 10:55Representative Results for Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
  • 11:29Conclusion

Summary

Automatic Translation

本文介绍了一种标准的方法来获得一个生物大分子的三维(3D)重建使用负染色电子显微镜(EM)的。在这个协议中,我们将解释如何使用随机的锥形倾斜重建方法(RCT)的决议中等酿酒酵母外切体复杂的三维结构。

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