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单粒子电子显微镜外切体综合重建,使用随机的锥形倾斜方法
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JoVE Journal
Biology
Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
Please note that all translations are automatically generated.
Click here for the English version.
单粒子电子显微镜外切体综合重建,使用随机的锥形倾斜方法
DOI:
10.3791/2574-v
•
12:10 min
•
March 28, 2011
•
Xueqi Liu
,
Hong-Wei Wang
1
Molecular Biophysics and Biochemistry
,
Yale University
Chapters
00:05
Title
01:11
Principle of the Random Conical Tilt Method
02:10
Preparation of Holey Carbon Grids Covered with Thin Carbon
05:08
Negative Staining of the Exosome Complex
06:23
Electron Microscopy of the Exosome Complex
08:10
Image Processing of the Data
10:55
Representative Results for Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
11:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种标准的方法来获得一个生物大分子的三维(3D)重建使用负染色电子显微镜(EM)的。在这个协议中,我们将解释如何使用随机的锥形倾斜重建方法(RCT)的决议中等酿酒酵母外切体复杂的三维结构。
Tags
Single Particle Electron Microscopy
EM Reconstruction
Exosome Complex
Random Conical Tilt Method
X-ray Crystallography
Macromolecular Complexes
Protein Sample
Negative Staining EM Method
Resolution
Symmetry
Heavy Metal Solution
Transmission Electron Microscope
2D Micrographs
Protein Molecules
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