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Chapters
- 00:05Title
- 01:26Isolation of Nuclei
- 03:07Isolation of Nucleosomes by in situ Micrococcal Nuclease Digestion
- 04:31Nucleosome-ELISA (NU-ELISA)
- 06:45Conclusion
Nucleosome ELISA (NU-ELISA) is a sensitive and quantitative method to detect global patterns of post-translational modifications in preparations of native, intact nucleosomes. These modifications include methylations, acetylations, and phosphorylations at specific histone amino acid residues, and hence NU-ELISA provides a global proteomic assay of the overall chromatin modification states of specific cell types.
