JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
偏光易位荧光蛋白
爪蟾
外胚层响应Wnt信号
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Polarized Translocation of Fluorescent Proteins in
Xenopus
Ectoderm in Response to Wnt Signaling
Please note that all translations are automatically generated.
Click here for the English version.
偏光易位荧光蛋白
爪蟾
外胚层响应Wnt信号
DOI:
10.3791/2700-v
•
06:55 min
•
May 26, 2011
•
Keiji Itoh
,
Sergei Y. Sokol
1
Department of Developmental and Regenerative Biology
,
Mount Sinai School of Medicine
Chapters
00:05
Title
01:00
Introduction
01:27
In vitro Fertilization of Xenopus Eggs
02:34
RNA Microinjection
03:46
Prepare Ectodermal Explants for Imaging
04:46
Alternative Approach: Prepare Cryosectioned Embryos for Imaging
05:57
Representative Fluorescent Imaging Results
06:29
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
非洲爪蟾胚胎外胚层已经成为一个有吸引力的模型研究细胞极性。描述一个试验,荧光蛋白的亚细胞分布在外胚层细胞进行评估。该协议将有助于地址问题涉及到空间的信号控制。
Tags
Polarized Translocation
Fluorescent Proteins
Xenopus Ectoderm
Wnt Signaling
Cell Polarity
Embryonic Development
Signaling Pathways
Molecular Components
Subcellular Localization
Protein Localization
RNA Microinjection
Ectodermal Explants
Epifluorescence
Diversin
Cytoplasmic Protein
Cell Polarity Determination
Wnt Signal Transduction
Wnt Ligand
Frizzled Receptor
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
电离辐射的反应定量的γH2AX灶
在活细胞荧光蛋白的双探头光学突出纯净的光转化的
膜片钳和灌注技术研究离子通道的表达
爪蟾
卵母细胞
在毛发再生的上皮 - 间充质信号的快速遗传分析
活体显微镜成像的亚细胞结构的活体小鼠表达荧光蛋白
在面部移植<em>非洲爪蟾</em>胚胎
该<em>爪蟾</em>卵母细胞切割开凡士林隙电压钳技术与荧光
重组β-catenin的降解<em>爪蟾</em>卵提取物
口面表型的定量<em>爪蟾</em
测量水润谷胱甘肽诱导饲养响应
Read Article