Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

Qiaozhi Wei; Nancy R. Manley; Brian G. Condie

doi:10.3791/2797

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Hele Mount In situ Hybridisatie van E8.5 aan E11.5 muis embryo's

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Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

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Hele Mount In situ Hybridisatie van E8.5 aan E11.5 muis embryo's

25,687 Views

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13:54 min

•

October 10, 2011

DOI:

10.3791/2797-v

DOI:

10.3791/2797-v

•

13:54 min

October 10, 2011

•

16 Views

Qiaozhi Wei¹, Nancy R. Manley¹, Brian G. Condie¹

¹Department of Genetics,University of Georgia

Summary

Deze hele mount In situ Hybridisatie protocol bespreekt kritische stappen die reproduceerbare resultaten van hoge kwaliteit voor genexpressie studies te waarborgen in E8.5-E11.5 dagen oude muis embryo's.

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Whole Mount In Situ Hybridization Gene Expression Patterns Embryos Probe Labeling Quality Control DIG-labeled RNA Probes In Vitro Transcription PCR Sensitivity Endogenous MRNA Fixation Storage Proteinase K Digestion Hybridization Conditions Post-hybridization Washes RNase Treatment Non-specific Probe Hybridization AP-conjugated Antibody

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Hele Mount<em> In situ</em> Hybridisatie van E8.5 aan E11.5 muis embryo's

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Summary

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