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全山原位杂交E8.5，E11.5小鼠胚胎

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Biology

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JoVE Journal Biology

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

Please note that all translations are automatically generated. Click here for the English version.

全山原位杂交E8.5，E11.5小鼠胚胎

DOI:

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13:54 min

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October 10, 2011

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Qiaozhi Wei, Nancy R. Manley, Brian G. Condie

¹Department of Genetics,University of Georgia

Chapters

00:05Title
01:09Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
03:58Collection of Mouse Embryos
05:12Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
10:07Antibody Detection of the Digoxygenin-labeled Riboprobe
12:38Examples of Whole-mount in situ Hybridizations
13:29Conclusion

Summary

Automatic Translation

这整个坐骑<em>原位</em>杂交协议的讨论，以确保在E8.5 - E11.5日龄小鼠胚胎的基因表达研究的重复性高品质的成果的关键步骤。

Tags

Whole Mount In Situ Hybridization Gene Expression Patterns Embryos Probe Labeling Quality Control DIG-labeled RNA Probes In Vitro Transcription PCR Sensitivity Endogenous MRNA Fixation Storage Proteinase K Digestion Hybridization Conditions Post-hybridization Washes RNase Treatment Non-specific Probe Hybridization AP-conjugated Antibody

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