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1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
Chapters
- 00:05Title
- 01:19Preparation for Surgery
- 02:19Laminectomy and Surgical Exposure of the L5 Dorsal Root
- 03:21Rhizotomy/Dorsal Root Crush
- 04:10Image Acquisition and Post-op Procedures
- 04:51Repeated Imaging
- 05:32Axonal Regeneration Following Dorsal Root Crush
- 06:16Conclusion
Bir<em> In vivo</em> Görüntüleme protokolü, dorsal kök ezmek aşağıdaki birincil duyusal aksonlar izlemek için tarif edilir. Prosedürleri geniş alan floresan mikroskopi ve thy1 YFP transgenik fareler kullanan ve akson rejenerasyonu PNS ve akson etkileşimleri CNS arayüzü ile 4 cm üzerinde tekrarlanan görüntüleme izni.
Tags
Live ImagingDorsal Root AxonsRhizotomySpinal Root InjuriesChronic PainSensory LossRegenerationDorsal Root Entry Zone (DREZ)Molecular EventsCellular EventsNerve InjuryPostmortem AnalysesAxon RegenerationReal-time EventsLiving AnimalNeurons Serially In VivoOpticsMouse TransgenicsThy1-GFP Transgenic MiceFluorescent ColorsIn Vivo ImagingMuscleBrainPhysiological MechanismsStatic AnalysesSpinal Cord Imaging
