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Kvantitativ analyse af Synaptic vesikel Pool Replenishment i dyrkede cerebellare Granule Neuroner bruger FM Farvestoffer
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Chapters
- 00:05Title
- 02:02Experimental Setup
- 02:36Sample Preparation
- 04:32S1 Phase
- 06:12Data Analysis
En live fluorescens billedbehandling teknik til at kvantificere opfyldning og mobilisering af specifikke synaptiske vesikel (SV) pools i det centrale nerve terminaler er beskrevet. To runder med SV genanvendelse overvåges i samme nerve terminalerne leverer en intern kontrol.
Tags
Quantitative AnalysisSynaptic Vesicle Pool ReplenishmentCultured Cerebellar Granule NeuronsFM DyesNeurotransmitter ReleaseEndocytosisRecycling PoolReadily Releasable PoolReserve PoolAssaySV TrafficClathrin-dependent EndocytosisActivity-dependent Bulk EndocytosisMobilisation MechanismsSV TurnoverCentral Nerve TerminalsHydrophobic Hydrocarbon TailHydrophilic Head GroupFluorescent Probes
