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Journal / Biology / En kvantitativ analys av insulin som uttrycker…
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Biology

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En kvantitativ analys av insulin som uttrycker kolonibildande stamfäder
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En kvantitativ analys av insulin som uttrycker kolonibildande stamfäder

Article DOI: 10.3791/3148-v • 14:39 min • November 28th, 2011
November 28th, 2011 •
1, 2, 2, 2, 2, 2, 2,3
1Department of Biotechnology & Bioinformatics, California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

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En tredimensionell klonogena analys som gör pankreas-liknande stamceller differentieras till insulinproducerande uttrycka kolonier beskrivs. Denna metod utnyttjar halvfasta media som innehåller metylcellulosa, Matrigel och tillväxtfaktorer, som enda stamceller förökar sig och differentiera

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Utvecklingsbiologi Pancreas insulin-uttryckande celler embryonala stamceller koloni analys progenitorceller 3-dimensionell kultur halvfasta medier Matrigel metylcellulosa
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