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NK细胞与干细胞技术的RosetteSep套件的人体血液的富集

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Biology

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JoVE Journal Biology

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

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NK细胞与干细胞技术的RosetteSep套件的人体血液的富集

DOI:

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11:52 min

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October 01, 2007

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Christine Beeton, K. George Chandy

¹Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapters

00:01Title
00:09Introduction
02:08Preparing Reagents
03:00Incubating Human Blood with RosetteSep NK Enrichment Cocktail
05:15Isolating NK Cells after Histopaque Density Gradient Centrifugation
11:07Conclusion

Summary

Automatic Translation

自然杀伤细胞是淋巴细胞的人口少。在这里，我们展示如何隔离这些细胞从人体血液中阴性选择，使用干细胞技术的试剂盒。获得的细胞是可行的和不变的抗体，因此准备了一些程序使用。

Tags

NK Cells RosetteSep Kit StemCell Technologies Positive Selection Negative Selection Surface Markers Peripheral Blood Cytotoxic Lymphocytes Innate Immune System Cancer Infections Pregnancy Transplant Rejection Antibody Cocktail Unwanted Cell Activation Differentiation Video-protocol

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