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通过显微注射介导的RNAi Necromenic线虫基因敲除和转基因 Pristionchus pacificus</em
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RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus
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通过显微注射介导的RNAi Necromenic线虫基因敲除和转基因 Pristionchus pacificus</em

DOI:
10.3791/3270-v

•

06:57 min

•

October 16, 2011

•

,
1Biology Department,California State University

Chapters

  • 00:05Title
  • 02:01DNA Preparation for Transgenesis
  • 02:51Microinjection into Hermaphroditic Gonads
  • 05:06Post-injection Storage & Screening
  • 05:45The P. pacificus Model
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

在模式生物，转基因操纵基因的功能，而RNAi能够敲除特定的mRNA转录1-2。该协议的目的是为了说明需要稳定传输的DNA和瞬态双链RNA引入necromenic线虫技术<em> Pristionchus pacificus</em在进化，发育和行为生物学的研究。

Tags

RNAi Mediated Gene KnockdownTransgenesisMicroinjectionNecromenic NematodePristionchus PacificusGene Function AnalysisExpression AnalysisCaenorhabditis ElegansTransgenic LinesNon-Caenorhabditis SpeciesNematodaStrongyloides StercoralisDevelopment StudyEvolution StudyBehavior StudyMicroinjection For TransgenesisGene Knockdown By RNAiGonads Of P. PacificusSyncitial GonadsTransgene InheritanceSomatic ExpressionSelf Genomic DNAEndonucleasesCoinjection Markers

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