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Chapters
- 00:05Title
- 02:01DNA Preparation for Transgenesis
- 02:51Microinjection into Hermaphroditic Gonads
- 05:06Post-injection Storage & Screening
- 05:45The P. pacificus Model
- 06:26Conclusion
In organismi modello, transgenesi in grado di manipolare le funzioni del gene, mentre RNAi può knockdown trascritti di mRNA specifico 1-2. Questo protocollo si propone di illustrare le tecniche necessarie per introdurre in modo stabile il DNA trasmessi e transitori di RNA a doppio filamento nel nematode necromenic<em> Pristionchus pacificus</em> Per gli studi in biologia evolutiva dello sviluppo, e comportamentali.
Tags
RNAi Mediated Gene KnockdownTransgenesisMicroinjectionNecromenic NematodePristionchus PacificusGene Function AnalysisExpression AnalysisCaenorhabditis ElegansTransgenic LinesNon-Caenorhabditis SpeciesNematodaStrongyloides StercoralisDevelopment StudyEvolution StudyBehavior StudyMicroinjection For TransgenesisGene Knockdown By RNAiGonads Of P. PacificusSyncitial GonadsTransgene InheritanceSomatic ExpressionSelf Genomic DNAEndonucleasesCoinjection Markers
