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Neuroscience
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人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Please note that all translations are automatically generated.
Click here for the English version.
人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导
DOI:
10.3791/3273-v
•
10:47 min
•
October 28, 2011
•
Xuejun H. Parsons
2
,
Yang D. Teng
4
,
James F. Parsons
2
,
Evan Y. Snyder
2,5
,
David B. Smotrich
2,6
,
Dennis A. Moore
2
1
San Diego Regenerative Medicine Institute
,
2
Xcelthera
,
3
Department of Neurosurgery
,
Harvard Medical School
,
4
Division of SCI Research, VA Boston Healthcare System
,
5
Program in Stem Cell & Regenerative Biology
,
Sanford-Burnham Medical Research Institute
,
6
La Jolla IVF
Chapters
00:05
Title
01:27
Media Preparation and Plate Coating
02:33
Passaging and Seeding Undifferentiated Human ES Cells
04:31
Neural Induction in Adhesive Culture with Retinoic Acid
05:42
Differentiation in Suspension Culture and Maturation to Neuronal Phenotype after Attachment
08:08
Direct Neuronal Differentiation of Feeder- and Serum- Free Pluripotent Human ES Cells WIth Retinoic Acid Induction
10:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经建立了诱导多能干人类胚胎干细胞与小分子物质,使推导的大量供应人类神经祖细胞和神经细胞类型,在发展中国家中枢神经系统神经修复定义的条件下保持直接的神经母细胞的协议。
Tags
Human Neuronal Progenitors
Neurons
Pluripotent Human Embryonic Stem Cells
Small Molecule Induction
Cell-based Therapies
Central Nervous System (CNS)
Neural Stem Cells
Expansion Ability
Plasticity
Trophic And Neuroprotective Molecules
Pluripotent HESCs
Neurological Disorders
Differentiation Potential
Developmental Study
Clinical Translation
Multi-lineage Inclination
Germ Layer
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