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Journal
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Immunology and Infection
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RNA的分离
绿脓杆菌
殖民小鼠胃肠道
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
RNA Isolation of
Pseudomonas aeruginosa
Colonizing the Murine Gastrointestinal Tract
Please note that all translations are automatically generated.
Click here for the English version.
RNA的分离
绿脓杆菌
殖民小鼠胃肠道
DOI:
10.3791/3293-v
•
08:52 min
•
September 28, 2011
•
Eduardo Lopez-Medina
,
Megan M. Neubauer
,
Gerald B. Pier
,
Andrew Y. Koh
1
Department of Pediatrics
,
University of Texas Southwestern Medical Center
,
2
Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital
,
Harvard Medical School
,
3
Department of Pediatrics and Microbiology
,
University of Texas Southwestern Medical Center
Chapters
00:05
Title
01:21
Harvesting Murine Luminal Contents
02:44
Bacterial RNA Isolation
06:03
DNAse Treatment
07:48
Representative Agilent Biolanalyzer Analysis of Murine Cecal Content-extracted Bacterial RNA
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
RNA提取的一种可靠的方法<em>绿脓杆菌</em从小鼠cecums恢复描述。回收的RNA是足够的数量和质量,为后续的qPCR,转录分析和RNA序列的实验。这种技术可以适用于RNA等肠道微生物的隔离。
Tags
RNA Isolation
Pseudomonas Aeruginosa
Gastrointestinal Tract
Colonization
Immune Systems
Leukemia
Burn Wounds
Organ Transplants
Bloodstream Infections
Transcription Profiling
MRNA
Gene Expression
Immune Status
Isolation
High Quality MRNA
Enzymes
Debris
Food Residues
Particulate Matter
GI Colonization
Neutropenia-induced Dissemination
Mice Euthanization
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