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慢性成人出生的嗅觉神经元的选择性病毒转导
在体内
Optogenetic刺激
JoVE Journal
Neuroscience
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Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic
in vivo
Optogenetic Stimulation
Please note that all translations are automatically generated.
Click here for the English version.
慢性成人出生的嗅觉神经元的选择性病毒转导
在体内
Optogenetic刺激
DOI:
10.3791/3380-v
•
12:00 min
•
December 28, 2011
•
Gabriel Lepousez
,
Mariana Alonso
,
Sebastian Wagner
,
Benjamin W. Gallarda
,
Pierre-Marie Lledo
1
Laboratory for Perception and Memory
,
Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)
Chapters
00:05
Title
01:35
Preparing the Microinjector
02:42
Preparing the Animal and Performing the Stereotaxic Injection
04:53
Preparing the Miniature LED for Implantation
06:54
Chronic LED Implantation for in vivo Optogenetic Stimulation
09:29
Injected Cells and Implantation
10:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
成人出生在嗅球的神经元可optogenetically控制使用Channelrhodopsin2表达慢病毒注射在延髓的迁徙流和植入微型慢性photostimulation的LED。
Tags
Selective Viral Transduction
Adult-born Olfactory Neurons
Chronic In Vivo Optogenetic Stimulation
Local Interneurons
Adult Neurogenesis
Lateral Ventricle
Rostral Migratory Stream (RMS)
Olfactory Bulb
Label And Manipulate Activity
Optogenetic Technologies
Channelrhodopsin2 (ChR2)-YFP
Temporally Restricted Cohort Of Neuroblasts
Implant And Calibrate Miniature LED
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