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High-throughput krystallisering af membranproteiner Brug af Lipidic Bicelle Metode
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JoVE Journal Biology
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

High-throughput krystallisering af membranproteiner Brug af Lipidic Bicelle Metode

DOI:
10.3791/3383-v

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07:26 min

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January 09, 2012

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,
1UCLA-DOE Institute for Genomics and Proteomics,University of California Los Angeles , 2Department of Physiology,David Geffen School of Medicine, UCLA

Chapters

  • 00:05Title
  • 01:13Preparation of Bicelles
  • 02:49Incorporation of Protein into Bicelles
  • 04:04Setting up Crystallization Trials
  • 06:07Representative Crystals
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

Bicelles er lipid / amphiphile blandinger, der opretholder membranproteiner (MP) inden for en tolagede men har unikke fase adfærd, der fremmer high-throughput screening ved krystallisation robotter. Denne teknik har med succes produceret en række høj opløsning strukturer fra både prokaryote og eukaryote kilder. Denne video beskriver protokoller for at generere lipidic bicelle blandingen, som omfatter parlamentsmedlemmer i bicelle blandingen, etablering krystalliseringer forsøg (såvel manuelt som robotersvejsede) og høste krystaller fra mediet.

Tags

High-throughput CrystallizationMembrane ProteinsLipidic Bicelle MethodHydrophobicityProtein-detergent MicelleSolubilize ProteinsCrystallization TrialsCrystal ContactsLipidic MediaLipidic Cubic Phase (LCP)

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