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Chapters
- 00:05Title
- 01:31Virus Production
- 03:53Viral Transduction
- 05:47Reprogramming
- 07:24Isolation of Human iPS Cell Colonies
- 11:14Representative Results: Examining Transduction Efficiency and Characterizing Reprogrammed Cells
- 12:58Conclusion
Vi presenterar ett protokoll för effektiv omprogrammering av mänskliga somatiska celler till humana inducerade pluripotenta stamceller (hiPSC) med retrovirusvektorer som kodar Oct3 / 4, Sox2, Klf4 och c-myc (OSKM) och identifiering av korrekt omprogrammeras hiPSC med levande färgning med Tra- 1-81 antikropp.
Tags
SelectingIsolatingColoniesHuman Induced Pluripotent Stem CellsReprogrammingAdult FibroblastsRetroviral VectorsOct3/4Sox2Klf4C-mycSodium ButyrateEfficient Transduction ProtocolVirus-containing MediaLive ImmunostainingTra-1-81Pluripotent CellsManually PassagedMatrigel PlatesFeeder-free ConditionsHES-like MorphologyExpansionCell LinesPluripotency MarkersTRA-1-60SSEA-4Oct3/4Sox2Nanog
