We describe a basic protocol for quantitating in vitro ATPase activity. This protocol can be optimized based on the level of activity and requirements for a given purified ATPase.
Rule, C. S., Patrick, M., Sandkvist, M. Measuring In Vitro ATPase Activity for Enzymatic Characterization. J. Vis. Exp. (114), e54305, doi:10.3791/54305 (2016).