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Biology
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哺乳动物链接组蛋白亚型表达分析
JoVE Journal
Biology
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JoVE Journal
Biology
Expression Analysis of Mammalian Linker-histone Subtypes
Please note that all translations are automatically generated.
Click here for the English version.
哺乳动物链接组蛋白亚型表达分析
DOI:
10.3791/3577-v
•
14:40 min
•
March 19, 2012
•
Magdalena Medrzycki
,
Yunzhe Zhang
,
Kaixiang Cao
,
Yuhong Fan
1
School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences
,
Georgia Institute of Technology
Chapters
00:05
Title
02:09
Sample Preparation and RNA Extraction
03:42
Quantitative Reverse Transcription PCR (qRT-PCR)
06:48
Preparation of Total Histones
09:48
HPLC Analysis of Linker Histones
10:53
Representative Results
13:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个集检测,分析H1链接组蛋白表达水平。个人H1基因的mRNA定量测定基于随机引物反转录实时PCR其次,而量化的H1组蛋白的蛋白质的高效液相色谱分析实现。
Tags
Linker-histone Subtypes
Linker Histone H1
Nucleosome Core Particle
Chromatin Structure
Gene Expression
Mammalian Development
H1 Subtypes
Somatic H1s
Germ Cell Specific H1 Subtypes
H1x5
DNA Binding Affinity
Chromatin Compaction Ability
Quantitative Expression Analysis
MRNA Expression
Reverse Transcription-PCR (qRT-PCR) Assays
Northern Blot Analysis
Histone Genes
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