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活细胞成像的移植细胞在一个三维矩阵表示荧光标记的蛋白质
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
DOI:

10:26 min

December 22, 2011

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Chapters

  • 00:05Title
  • 01:00Generation of a Stable Cell Line
  • 02:50Silanize the Glass-bottom Dish
  • 04:03Preparation of 3D-collagen Gel With Tracer Particles
  • 05:35Time-lapse Image Acquisition
  • 06:29Inhibitor-addition Experiments
  • 07:11FRAP Procedure and Analysis
  • 08:15Following Proteins in 3D Cell Migration
  • 10:06Conclusion

Summary

Automatic Translation

传统的细胞过程,如细胞迁移研究二维的,僵硬的塑料表面上。本报告介绍了一个直接可视化蛋白定位和分析一个有关生理,立体矩阵的细胞迁移的蛋白质动力学的技术。

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