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Bioengineering
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活细胞成像的移植细胞在一个三维矩阵表示荧光标记的蛋白质
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
Please note that all translations are automatically generated.
Click here for the English version.
活细胞成像的移植细胞在一个三维矩阵表示荧光标记的蛋白质
DOI:
10.3791/3589-v
•
10:26 min
•
December 22, 2011
•
Wenting Shih
,
Soichiro Yamada
1
University of California, Davis
Chapters
00:05
Title
01:00
Generation of a Stable Cell Line
02:50
Silanize the Glass-bottom Dish
04:03
Preparation of 3D-collagen Gel With Tracer Particles
05:35
Time-lapse Image Acquisition
06:29
Inhibitor-addition Experiments
07:11
FRAP Procedure and Analysis
08:15
Following Proteins in 3D Cell Migration
10:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
传统的细胞过程,如细胞迁移研究二维的,僵硬的塑料表面上。本报告介绍了一个直接可视化蛋白定位和分析一个有关生理,立体矩阵的细胞迁移的蛋白质动力学的技术。
Tags
Live-cell Imaging
Migrating Cells
Fluorescently-tagged Proteins
Three-dimensional Matrix
Cell Migration
Biological Processes
Wound Healing
Cancer Metastasis
Protein Localization
Protein Dynamics
Extracellular Matrix
Optically Clear Matrix
Protein Functions
Genetically Encoded Fluorescent Tags
Subcellular Accumulation
Cytoskeletal Components
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