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酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
Please note that all translations are automatically generated.
Click here for the English version.
酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析
DOI:
10.3791/3602-v
•
07:15 min
•
November 26, 2011
•
Yuan-Hao Hsu
,
Jolinda A. Traugh
1
Department of Chemistry
,
Tunghai University
,
2
Department of Biochemistry
,
University of California, Riverside
Chapters
00:05
Title
02:14
Identification of Pepsin-digested Pak2 Fragments
03:34
Measurement of Amide H/D Exchange
05:30
Time Course of Deuterium Incorporation for Inactive Pak2
06:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
成功地利用MALDI - TOF质谱监察PAK2活化蛋白激酶的酰胺氢/氘交换。
Tags
Amide Hydrogen/deuterium Exchange
MALDI-TOF Mass Spectrometry
Protein-protein Interactions
Protein Conformational Changes
Protein Dynamics
Protein-ligand Interactions
Deuteration Rates
Pepsin Digestion
Resolution
Heteronuclear Single Quantum Coherence (HSQC)
NMR
Aqueous Solution
MALDI-TOF Detection
HPLC/ESI-MS System
Protein Kinase Pak2
Gamma-Pak
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