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表型分析和分离小鼠造血干细胞和传承的承诺祖
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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Please note that all translations are automatically generated.
Click here for the English version.
表型分析和分离小鼠造血干细胞和传承的承诺祖
DOI:
10.3791/3736-v
•
12:03 min
•
July 08, 2012
•
Michela Frascoli
,
Michele Proietti
,
Fabio Grassi
2
1
Institute for Research in Biomedicine
,
Bellinzona (Switzerland)
,
2
Dipartimento di Biologia e Genetica per le Scienze Mediche
,
Universitá degli Studi di Milano
Chapters
00:05
Title
01:45
Preparation of Cell Suspension from Bone Marrow
03:49
Phenotypic Analysis
06:00
Magnetic Enrichment of c-Kit+ Cells
08:11
Representative Results
11:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述的方法来分析流式细胞仪检测骨髓造血祖细胞的分布以及有效地隔离高度纯化造血干细胞(HSCs)。基本上是基于C-kit +细胞和排序,以净化造血干细胞和分子生物学研究细胞的磁富集分离过程。
Tags
Phenotypic Analysis
Isolation
Murine Hematopoietic Stem Cells
Lineage-committed Progenitors
Bone Marrow
HSCs
Blood Cell Lineages
Molecular Dissection
Homeostasis
Hematopoiesis
Oncology
Regenerative Medicine
Labeling Protocol
Fluorescent Antibodies
Electronic Gating Procedure
Flow Cytometry
Hematopoietic Progenitor Subsets
Distribution
Individual Mice
Magnetic Enrichment
C-Kit Expression
Trabecular Osteoblasts
Sinusoidal Endothelium
Functional Niches
N-cadherin+ Osteoblasts
Receptor Tyrosine Kinase Tie2
Angiopoietin-1
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