Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Shan Zong; Shuyun Deng; Kenian Chen; Jia Qian Wu

doi:10.3791/52104

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

JoVE Journal
Biology

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JoVE Journal Biology

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

12,276 Views

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12:44 min

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November 11, 2014

DOI:

10.3791/52104-v

DOI:

10.3791/52104-v

•

12:44 min

November 11, 2014

•

9 Views

Shan Zong*¹, Shuyun Deng*¹, Kenian Chen¹, Jia Qian Wu¹

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center,The University of Texas Graduate School of Biomedical Sciences at Houston

Summary

RNA-sequencing and bioinformatics analyses were used to identify significantly and differentially expressed transcription factors in Lin-CD34+ and Lin-CD34- subpopulations of mouse EMLcells. These transcription factors might play important roles in determining the switch between self-renewing Lin-CD34+ and partially differentiated Lin-CD34- cells.