Journal
/
Immunology and Infection
/
定量成像小样本人体血液中单核细胞和树突状细胞系特定的Toll样受体介导的信号
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Please note that all translations are automatically generated. Click here for the English version.

定量成像小样本人体血液中单核细胞和树突状细胞系特定的Toll样受体介导的信号

DOI:
10.3791/3741-v

•

07:58 min

•

April 16, 2012

•

,
1Department of Internal Medicine,Yale University School of Medicine

Chapters

  • 00:05Title
  • 01:45Isolation, Stimulation, and Labeling of Immune Cells
  • 03:48ImageStream Analysis
  • 05:59Results: Representative Images of NFkB Translocation in Specific mDCs-lineage Cell Groups in Response to TLR Signaling
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述ImageStream技术的使用（<a href="http://www.amnis.com/" target="_blank"> www.amnis.com</a>），它结合了定量流式细胞仪同时高分辨率数字成像，量化定义良好的病人同伙的主要免疫细胞的细胞机制。我们的研究提供了一个蓝图转化调查量化谱系在小样本的具体细胞的反应，受同伙。

Tags

Quantitative ImagingLineage-specificToll-like Receptor-mediated SignalingMonocytesDendritic CellsSmall SamplesHuman BloodImmune StatusInfectionDisease SeverityOutcomeAgingViral InfectionsBacterial InfectionsVaccinesHumoral Immune ResponsesCell-mediated Immune ResponsesToll-like Receptors (TLRs)Innate Immune SystemAntimicrobial Host Defense ResponsesPeripheral Blood MonocytesElderly DonorsDendritic Cells (DCs)Antigen-presenting CellsInnate ImmunityAdaptive ImmunityDysregulation Of TLR3MacrophagesIFN ProductionWest Nile Virus InfectionImmunosenescenceTherapeutic Intervention

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3741?article_type=v

在调节Toll样受体激活Neu1唾液酸酶活动的检测
check_url/3741?article_type=v

从人类胸腺髓样树突状细胞和上皮细胞的分离
check_url/3741?article_type=v

从外周血人类同种异体抗原特异性T细胞的生成
check_url/3741?article_type=v

从全血人单核细胞来源的树突状细胞的生成
check_url/3741?article_type=v

单核细胞单层试验中用于评估Fcγ受体介导的吞噬作用
check_url/3741?article_type=v

葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率
check_url/3741?article_type=v

为了应对抑菌活性的实时成像人力初级中性粒细胞和单核细胞<em>一种。曲霉</em
check_url/3741?article_type=v

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
check_url/3741?article_type=v

人雌性生殖道树突状细胞表型鉴定的分离及功能研究
check_url/3741?article_type=v

人干扰素α超灵敏单分子阵列数字酶联免疫吸附试验的研制与验证

Read Article