/
/
TransFLP - שיטה גנטית לשינוי<em> Vibrio cholerae</em> בהתבסס על שינוי הטבעי וFLP-רקומבינציה
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:16Preparation of Chitin Flakes
- 01:39Oligonucleotide Design and Polymerase Chain Reaction
- 03:45Chitin-induced Natural Transformation
- 04:59Removal of Selective Cassette(s) by FLP-recombination
- 05:46Plasmid Curing
- 06:21Results: Representative TransFLP Modifications of V. cholerae
- 11:34Conclusion
Tags
TransFLPGenetically ModifyVibrio CholeraeNatural TransformationFLP-recombinationBacterial ChromosomesReplicative PlasmidsHomology-mediated RecombinationInsertional MutantsPlasmid ExcisionGram-negative BacteriaLevan Sucrase EnzymeSacB GenePre-insertion GenotypeModified GeneTime-consumingCloningHorizontal TransferArtificial TransformationPositive SelectionRapid ManipulationChitin-mediated InductionNatural Competence
