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Immunology and Infection
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TransFLP - A基因修改方法<em>霍乱弧菌</em基于自然转化和FLP重组
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Immunology and Infection
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JoVE Journal
Immunology and Infection
TransFLP — A Method to Genetically Modify
Vibrio cholerae
Based on Natural Transformation and FLP-recombination
Please note that all translations are automatically generated.
Click here for the English version.
TransFLP - A基因修改方法<em>霍乱弧菌</em基于自然转化和FLP重组
DOI:
10.3791/3761-v
•
12:13 min
•
October 08, 2012
•
Melanie Blokesch
1
Global Health Institute, School of Life Sciences
,
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Chapters
00:05
Title
01:16
Preparation of Chitin Flakes
01:39
Oligonucleotide Design and Polymerase Chain Reaction
03:45
Chitin-induced Natural Transformation
04:59
Removal of Selective Cassette(s) by FLP-recombination
05:46
Plasmid Curing
06:21
Results: Representative TransFLP Modifications of
V. cholerae
11:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个快速的方法来修改的基因组<em> V.霍乱</em>进行说明。这些修改包括删除单个基因,基因簇和基因组岛屿以及集成的短序列(如启动子元件或亲和标签序列)。该方法是基于对自然的改造和FLP重组。
Tags
TransFLP
Genetically Modify
Vibrio Cholerae
Natural Transformation
FLP-recombination
Bacterial Chromosomes
Replicative Plasmids
Homology-mediated Recombination
Insertional Mutants
Plasmid Excision
Gram-negative Bacteria
Levan Sucrase Enzyme
SacB Gene
Pre-insertion Genotype
Modified Gene
Time-consuming
Cloning
Horizontal Transfer
Artificial Transformation
Positive Selection
Rapid Manipulation
Chitin-mediated Induction
Natural Competence
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