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龙虎斗动粒蛋白的动态行为与染色体运动,在生活学习有丝分裂关卡<em>果蝇</em>合胞胚胎
JoVE Journal
Biology
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JoVE Journal
Biology
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living
Drosophila
Syncytial Embryos
Please note that all translations are automatically generated.
Click here for the English version.
龙虎斗动粒蛋白的动态行为与染色体运动,在生活学习有丝分裂关卡<em>果蝇</em>合胞胚胎
DOI:
10.3791/3763-v
•
13:59 min
•
June 14, 2012
•
Maureen Sinclair
,
Jun-Yong Huang
1
Institute for Cell and Molecular Biosciences
,
University of Newcastle, United Kingdom
Chapters
00:05
Title
01:45
Maintaining Flies and Collecting Eggs
03:43
Preparing Coverslips and Slides
05:10
Dechorionating Embryos
06:36
Provoking the SAC by Microinjecting Embryos with Appropriate Reagents
08:55
Imaging Embryos
10:08
Results: Observing Dynamic Kinetochore Behavior and Chromosome Motion
13:28
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
着丝粒是地方国资委启动其信号监测的有丝分裂的姐妹染色单体隔离。一种方法是描述与染色体运动的可视化招聘和营业额的着丝粒蛋白及其协调<em>果蝇</em>胚胎使用徕卡激光扫描共聚焦系统。
Tags
Mitotic Checkpoint
Kinetochore Protein
Chromosome Motion
Living Drosophila Syncytial Embryos
SAC Mechanism
Aneuploidy
Genomic Instability
Cancers
Birth Defects
Alzheimer’s
Mad1
Mad2
Bub1
BubR1
Bub3
Mps1
Zw10
Rod
Aurora B Kinase
Cdc20
APC/C Activators
Cyclin B
Securin
Metaphase To Anaphase Transition
SAC Signal Initiation
Kinetochore Assembly
Relay Onto APC/C Inhibition
Drosophila Experimental System
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