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放射性的原位杂交检测组织不同的基因表达模式
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
DOI:

17:38 min

April 27, 2012

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Chapters

  • 00:05Title
  • 02:16Tissue Preparation
  • 03:20Pre-hybridization Treatment of Tissue
  • 04:36Probe Hybridization
  • 07:34Visualization of the Radioactive Signal
  • 10:59Generating Darkfield Color Images
  • 11:38Results: mRNA Expression Patterns in Avian Brain
  • 16:56Conclusion

Summary

Automatic Translation

该协议被成功地用于定量检测水平和mRNA表达的空间格局,在跨多个组织的脊椎动物物种类型。该方法可以检测低丰度的成绩单,并允许数以百计的幻灯片,同时处理。我们目前使用的禽流感为例,胚胎的大脑形成的表达分析这个协议。

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