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单细胞的多巴胺释放,同时电压钳和电流分析法测量
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
DOI:

07:30 min

November 21, 2012

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Chapters

  • 00:05Title
  • 01:22Equipment and Electrode Preparation
  • 04:05Preparation of Dopamine Neuronal Culture and Patch-clamp Recording
  • 06:12Results: Whole-cell and Amperometric Recording at Multiple Voltages
  • 06:46Conclusion

Summary

Automatic Translation

电化学技术措施,通过检测自发多巴胺的氧化产生的氧化电流从一个单细胞多巴胺的释放。同时钳位电压和安培的方法揭示了整体的“活动”,这种活动对多巴胺的反向运输的多巴胺转运体的调节作用机理之间的关系。

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