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Neuroscience

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JoVE Journal Neuroscience
Encelliga Mätning av dopaminfrisättning med samtidig Spänning-klämma och amperometri
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Encelliga Mätning av dopaminfrisättning med samtidig Spänning-klämma och amperometri

Article DOI: 10.3791/3798
November 21st, 2012 •
1, 1, 1,2
1Department of Neuroscience, University of Florida, 2Department of Psychiatry, University of Florida

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November 21st, 2012

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Den amperometriska tekniken mäter dopaminfrisättning från en enda cell genom att detektera den oxidativa ström som produceras av spontan dopamin oxidation. Samtidig spänning klämma och amperometri metodik avslöjar mekanistiska förhållandet mellan den totala "aktivitet" av dopamin transportören och reglerande roll denna aktivitet på baksidan transport av dopamin.

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