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人类体细胞重编程为诱导多能干细胞(iPS细胞)用绿色荧光蛋白逆转录病毒载体
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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
Please note that all translations are automatically generated.
Click here for the English version.
人类体细胞重编程为诱导多能干细胞(iPS细胞)用绿色荧光蛋白逆转录病毒载体
DOI:
10.3791/3804-v
•
08:25 min
•
April 03, 2012
•
Kun-Yong Kim
,
Eriona Hysolli
,
In-Hyun Park
1
Yale Stem Cell Center, Department of Genetics
,
Yale School of Medicine
Chapters
00:05
Title
01:28
Infecting Cells with Retrovirus Expression Reprogramming Factors
03:31
Isolation and Expansion of Induced Pluripotent Stem Cells
04:42
Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
06:06
Representative Cell-reprogramming Results
08:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
的方法来产生人类诱导多能干细胞(iPS细胞)通过逆转录病毒介导的异位表达Oct4的,SOX2的,KLF4和MYC的描述。基于GFP的表达,以确定人类IPSC的殖民地一个切实可行的办法进行了讨论。
Tags
Reprogramming
Human Somatic Cells
Induced Pluripotent Stem Cells
Retroviral Vector
GFP
Human Embryonic Stem Cells
Pluripotent
In Vitro Disease Modeling
Regenerative Medicine
Transcription Factors
Oct4
Sox2
Klf4
Myc
Autologous Cells
Fibroblast Cells
GFP-containing Retroviral Backbone
ESC Culture Condition
Morphology
Retroviral Transgene Silencing
Fluorescence Microscope
Pluripotency Genes
Surface Markers
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