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一个荧光识别GIRK通道调制器的筛选试验
JoVE Journal
Biology
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JoVE Journal Biology
A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
DOI:

05:31 min

April 24, 2012

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Chapters

  • 00:05Title
  • 01:20Preparation of the Cells, Test Compounds and Cell Treatment
  • 03:05Activation of GIRK Channels: Fluorescent Measurement and Analysis
  • 04:10Results: Fluorescence Signal Measured using the GIRK Channel Assay
  • 05:07Conclusion

Summary

Automatic Translation

一个确定药物相互作用与G蛋白门控内向整流钾实时甄别<sup> -</sup>(GIRK)通道描述。该法采用膜电位敏感荧光染料来衡量GIRK通道活性。这项技术是适应的细胞株上使用。

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