JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
一个荧光识别GIRK通道调制器的筛选试验
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
Please note that all translations are automatically generated.
Click here for the English version.
一个荧光识别GIRK通道调制器的筛选试验
DOI:
10.3791/3850-v
•
05:31 min
•
April 24, 2012
•
Maribel Vazquez
,
Charity A. Dunn
,
Kenneth B. Walsh
1
Department of Pharmacology, Physiology & Neuroscience
,
University of South Carolina, School of Medicine
Chapters
00:05
Title
01:20
Preparation of the Cells, Test Compounds and Cell Treatment
03:05
Activation of GIRK Channels: Fluorescent Measurement and Analysis
04:10
Results: Fluorescence Signal Measured using the GIRK Channel Assay
05:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
一个确定药物相互作用与G蛋白门控内向整流钾实时甄别<sup> -</sup>(GIRK)通道描述。该法采用膜电位敏感荧光染料来衡量GIRK通道活性。这项技术是适应的细胞株上使用。
Tags
Fluorescent Screening Assay
Modulators
GIRK Channels
G Protein-gated Inward Rectifier K+
Cellular Mediators
Hormones
Neurotransmitters
Brain
Heart
Skeletal Muscle
Ligands
Plasma Membrane-bound Receptors
G Protein-coupled Receptors (GPCRs)
Stimulation Of G Proteins
Gi And Go
K+ Movement Out Of The Cell
Resting Membrane Potential
Neurons
Action Potential Formation
Excitatory Neurotransmitters
Heart Rate
Therapeutic Agents
Neuropathic Pain
Drug Addiction
Cardiac Arrhythmias
Pharmacology
Anti-arrhythmic Agents
Antipsychotic Drugs
Antidepressants
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
异源表达在哺乳动物细胞离子通道的突变和功能分析
高通量筛选和生物传感与荧光
C。线虫
菌株
转换到一个点阵仪XMAP含量,使用多重抗体筛查方法的捕获ELISA
内向整流钾通道的小分子调节剂的高通量筛选
使用自制双辉光萤光素酶检测的高通量筛选功能
膜蛋白的绿色荧光蛋白为基础的表达筛选<em>大肠杆菌</em
一个脂蛋白体为基础的外排检测,以确定氯的单分子性质<sup> -</sup>通道和转运
遗传条形码与荧光蛋白的复用的应用程序
斑马鱼肾功能评价采用荧光测定间隙
高通量筛选转录后调控基因的化学调节剂
Read Article