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Um método passo-a-passo para a reconstituição de um transportador ABC em partículas lipídicas Nanodisc

Article DOI: 10.3791/3910-v • 12:25 min • August 31st, 2012
August 31st, 2012 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of British Columbia

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Nanodiscs são pequenas partículas discóides que incorporam proteínas de membrana em um pequeno pedaço de bicamada de fosfolipídios. Nós fornecemos um protocolo visual que mostra a incorporação passo-a-passo do transportador MalFGK2 em um disco.

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Questão 66 Nanodiscs proteínas de membrana lipídios transportadores ABC e dos transportadores de maltose MalFGK
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