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Biology
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在油脂中间相结构的测定生物大分子晶体生长的膜蛋白晶体收获和低温冷
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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
Please note that all translations are automatically generated.
Click here for the English version.
在油脂中间相结构的测定生物大分子晶体生长的膜蛋白晶体收获和低温冷
DOI:
10.3791/4001-v
•
18:45 min
•
September 02, 2012
•
Dianfan Li
,
Coilín Boland
,
David Aragao
,
Kilian Walsh
,
Martin Caffrey
1
Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology
,
Trinity College Dublin
Chapters
00:00
Title
00:29
Materials and Equipment
02:12
Identifying Wells With Crystals
03:13
Opening the Well
04:04
“Closeup”: Opening Wells with Cubic Phase – Method I
06:38
“Closeup”: Opening Wells with Cubic Phase – Method 2
08:27
Opening Well with Sponge Phase – Unsuccessfully
10:25
Opening Well with Sponge Phase – Successfully
14:20
Harvesting Crystals
15:16
Harvesting Crystals from the Cubic Phase
16:12
Harvesting Crystals from the Sponge Phase
16:40
Cryo-Cooling Crystals
17:25
Storage/Transport Dewars
17:42
Crystal Images
18:03
Concluding Remarks
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文所描述的过程中实现卡弗里膜的结构和功能的生物集团收获和低温冷膜蛋白晶体生长大分子的X射线晶体结构测定中使用的油脂的立方和海绵阶段。
Tags
Harvesting
Cryo-cooling
Crystals
Membrane Proteins
Lipidic Mesophases
Structure Determination
Macromolecular Crystallography
Integral Membrane Proteins
Macromolecular X-ray Crystallography
MX Diffraction Quality Crystals
G Protein-coupled Receptor Field
In Meso Method
Lipidic Cubic Phase Method
Technical Challenges
Viscous Nature
Sticky Nature
Micro-crystals
Manipulating Crystals
Glass Sandwich Plates
Article
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