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Immunology and Infection
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Analyse av Pulmonal dendrittiske celle modning og migrasjon under Allergisk Airway betennelse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Analyse av Pulmonal dendrittiske celle modning og migrasjon under Allergisk Airway betennelse

DOI:
10.3791/4014-v

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07:52 min

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July 23, 2012

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,
1Stem Cell and Cancer Research Institute,McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children,University of Toronto

Chapters

  • 00:05Title
  • 01:24Sensitizing and Intranasal Challenge of Mice with Ovalbumin
  • 02:39Preparation of Single-cell Suspension from the Lungs and the Draining Lymph Nodes
  • 05:37Results: OVA Sensitization and Challenge Induces an Increase in CD11c+ Cell Trafficking to Murine Mediastinal Lymph Nodes
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en strategi for å overvåke modning og migrasjon av lunge dendrittiske celler som reaksjon på ovalbumin i innstillingen av ovalbumin indusert allergisk Airway betennelse. Denne strategien kan endres for å vurdere migrasjon av pulmonale dendrittiske celler i innstillingene for infeksjon.

Tags

Pulmonary Dendritic CellsMaturationMigrationAllergic Airway InflammationAdaptive Immune ResponseAntigen-specific T CellsAntigen StimulationDraining Lymph NodesT Cell ResponseAutoimmune ResponseAllergic Immune ResponseImmune ToleranceLung EnvironmentEnvironmental AntigensAllergyAsthmaAirway DC MaturationAirway DC MigrationSensitizationOvalbumin-induced Allergic Asthma

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